ANTIINFLAMMATORY ACTIVITY TEST
Inflammatory diseases are characterised by a
continuous recruitment of white blood cells (leucocytes) to site of injuries,
infections or antigen depositions. It is now well established that the key step
in the recruitment of white blood cells at inflammatory sites is the
adhesive interaction of white blood cells with the vascular endothelium
(endothelial cells) and/or extracellular matrix proteins. A number of
potent inflammatory disease mediators including cytokines (e.g. TNF, IL-1 and
IL-6) and prostaglandins have also been shown to up-regulate the expression of
various endothelial and white blood cell surface adhesive molecules leading to
cell-cell or cell-matrix interactions and white blood cell immigration to
During the past sixteen years, we have
developed and optimised a number of in vitro cell and protein-based
assays that measure the potential antiinflammatory activity of herbal-products
against the above mentioned novel inflammatory targets. Some of these
established assays are listed below:
Endotoxin binding/detoxification tests
Cytokines production and release assays
Cell-cell adhesion (e.g. leucocyte-endothelial adhesions)
Cell-matrix interactions (e.g. leucocytes-protein adhesions)
Adhesion molecules expression and/or activation assays
Various experimental models of inflammation
Our review articles describe the
various approaches in antiinflammatory activity
studies of natural products:
HABTEMARIAM, S. (2010).
Applying new science for old medicines:
targeting leukocyte-endothelial adhesions by
antiiflammatory herbal drugs.
5(8), 1329-1336. Review
HABTEMARIAM S. (2013). Targeting the production
of monocytes/macrophages-derived cytokines by
anti-inflammatory herbal drugs.
Free Full Text Article
further information on the
subject or collaborative and/or consultancy work please
site owner. Our key publications below can also be used for quick
references on this area of interest:
Belai, A. (2017)
Natural Therapies of the inflammatory bowel
disease: The case of
rutin and its aglycone, quercetin.
Mini Review in Medicinal Chemistry.
Going back to the
good old days: The merit of crude plant
drug mixtures in the 21st
International Journal of Complementary &
E.K., Annan, K., Dickson, R.A., Mensah, A.Y., Amponsah, I.K., Appiah,
A.A., Tung, N.H., Edoh, D.A., HABTEMARIAM, S.* (2017).
Antiinflammatory and analgesic effects in rodent models of
ethanol extract of Clausena anisata
roots and their chemical constituents.
HABTEMARIAM, S. (2016). Berberine and
inflammatory bowel disease: A concise review.
113(Part A), 592-599.
Armah, F.A., Annan,
K, Mensah, A.Y., Amponsah, I.K., Tocher, D.A.,
HABTEMARIAM, S.* (2015). Erythroivorensin A:
a novel antiifnlammatory diterpene
from the root-bark of Erythrophleum
ivorense (A Chev.). Fitoterapia
Mensah, A.Y., Mensah, M.L.K., Tocher, D.A.,
HABTEMARIAM, S.*. (2014).
Anti-inflammatory properties of the stem-bark of Anopyxis
kalineana and its major constituent, methyl angolensate.
HABTEMARIAM S., Simplice, F.H., Thiery B.N.M.,
Abraham, J.A., Theophile, D. (2014).
Topically applied Tetrapleura
bark extract promotes healing of
excision and incision wounds in rats.
Journal of Intercultural Ethnopharmacology
HABTEMARIAM, S. (2010). Applying new science for old medicines: targeting
leukocyte-endothelial adhesions by antiiflammatory herbal drugs. Natural
Houghton P.J., HABTEMARIAM,
S., Kéry Á. (2009).
Antioxidant and antiinflammatory effect of Epilobium parviflorum
Phytother. Res. 23(5), 719-724.
Motlhanka, D.M.T. and HABTEMARIAM, S. (2007). Prostaglandin
E-2 (PGE-2) inhibition by crude extracts of medicinal plants of Botswana.
Nigerian Journal of Natural Products and Medicine 11, 32-33.
Hamamelis virginiana inhibits
the tumour necrosis factor-a
(TNF)-induced endothelial cell death in vitro.
HABTEMARIAM, S. (2001).
Antiinflammatory activity of
the antirheumatic herbal drug, gravel root (Eupatorium
purpureum): further biological activities and
constituents. Phytotherapy Res.
S. (2000). Natural inhibitors of tumour necrosis
production, secretion and function.
HABTEMARIAM, S. (1998).
Cistifolin, an integrin-dependent
cell adhesion blocker from the anti-rheumatic herbal
drug, gravel root (rhyzome of
Planta Med. 683-685.
HABTEMARIAM, S. (1998). Extract of gravel root
Eupatorium purpureum) inhibits
integrins-dependent U937 cell adhesion.
Res. 12, 422-426.
HABTEMARIAM, S. (1998).
Andrographolide inhibits the
tumour necrosis factor-a
induced upregulation of ICAM-1 expression and
Extract of corn silk (stigma of
inhibits the TNF and Bacterial Lipopolysacharide-induced
cell adhesion and ICAM-1 expression.
HABTEMARIAM, S. (1997).
inhibitors or enhancers of the cytotoxicity of tumor
necrosis factor-alpha in L-929 tumor cells.
J. Nat Prod.
60, 775-778.Modulation of
tumour necrosis factor-a-induced
cytotoxicity by polyphenols
Modulation of tumour necrosis factor-a-induced
cytotoxicity by polyphenols.
Cytotoxicity and immunosuppressive activity of
withanolides from Discopodium penninervium. Planta
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The Site Owner
Dr Habtemariam BSc, MSc,
PhD........FRSM, FRSC. .... With a
Principal Lecturer post at Greenwich, Dr Habtemariam
is a leader of the BSc Pharmaceutical Science programme and
researches on bioassay & natural products-based drug
addition to our fully equipped tissue culture
facilities, we have access to various
state-of-the-art equipment including ICP-MS, LC-MS,
MALDI-TOF-MS, FABS-MS, FTIR, Laser-Raman,
scanning and transmission electron
microscopes, flow cytometer, NMR (270, 300
and 500MHz), automated DNA sequencers, various HPLC
systems, capillary electrophersis and ABI PRCmate DNA