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ANTIINFLAMMATORY ACTIVITY TEST
Inflammatory diseases are characterised by a
continuous recruitment of white blood cells (leucocytes) to site of injuries,
infections or antigen depositions. It is now well established that the key step
in the recruitment of white blood cells at inflammatory sites is the
adhesive interaction of white blood cells with the vascular endothelium
(endothelial cells) and/or extracellular matrix proteins. A number of
potent inflammatory disease mediators including cytokines (e.g. TNF, IL-1 and
IL-6) and prostaglandins have also been shown to up-regulate the expression of
various endothelial and white blood cell surface adhesive molecules leading to
cell-cell or cell-matrix interactions and white blood cell immigration to
inflammatory sites.
During the past sixteen years, we have
developed and optimised a number of in vitro cell and protein-based
assays that measure the potential antiinflammatory activity of herbal-products
against the above mentioned novel inflammatory targets. Some of these
established assays are listed below:
Endotoxin binding/detoxification tests
Cytokines production and release assays
COX-products
release assay
Cell-cell adhesion (e.g. leucocyte-endothelial adhesions)
Cell-matrix interactions (e.g. leucocytes-protein adhesions)
Adhesion molecules expression and/or activation assays
Various experimental models of inflammation
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Our recent review article describes the
various approaches in antiinflammatory activity
studies of natural products:
HABTEMARIAM, S. (2010).
Applying new science for old medicines:
targeting leukocyte-endothelial adhesions by
antiiflammatory herbal drugs.
Natural
Product Communications
5(8), 1329-1336. Review
article -
Abstract |
For any
further information on the
subject or collaborative and/or consultancy work please
contact the
site owner. Our key publications below can also be used for quick
references on this area of interest:
Habtemariam S
Habtemariam S
Habtemariam S
Habtemariam S
Habtemariam S
Please also visit our publication page
for more references.
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