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HERBAL ANALYSIS: SPECTROSCOPY
Spectroscopy serves as the HERBAL ANALYST eyes, ears and nose.
If you don’t know what plant-derived product you have or working on, we advise
you not to take anyone’s word but to get its SPECTRUM! We have a range of
spectroscopic techniques at our deposal: ultraviolet, infrared, mass and NMR
spectroscopy are just few to mention. Below is a simplified summary of
combined spectroscopic techniques that we may apply to identify an unknown
plant-derived product:
By using NMR spectroscopy, we look at bonding atoms through their nuclei.
We establish connectivity of atoms through bonds, spatial arrangement of atoms,
and 1:1 correspondences between signals and atoms. Click
here for some guidance on NMR data interpretation.
Through Mass spectroscopy, we establish not only the molecular weight of
the compound in question but also distinguishes rival possibilities through
controllable fragmentation experiments. Click
here
for some guidance on mass spectral data interpretation.
By measuring
vibrational characteristics of bonds, functional groups of the molecule/compound
are identified using Infrared spectroscopy. This may also be used as a
'fingerprint' for routine analysis of products identity, purity, etc.
Ultraviolet spectroscopy confirms conjugation and
multiple bonds in a molecule.
The above spectroscopic
techniques are often combined with chromatography for simultaneous separation
and structural identifications. We also use spectroscopic techniques in
various bioassays to study, drug-drug or drug-biological target interactions.
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