High Performance Liquid Chromatography

(H P L C)

This is one of the most widely used chromatographic method for herbal drugs standardisation, quality control analysis, purification of compounds and even structural confirmations. The various modes of chromatography available for  HPLC systems,  applications and recent advances in HPLC methods-based method  development are covered in our lecture series.

We keep adding new equipment to our established stock of state-of- the-art facilities. The picture on the left is a new addition this year that is  solely dedicated  for routine standardisation of natural products. We are particularly keen in standardisations based on known bioactive compounds or markers. Once a suitable method for the limit of detection, limit of quantification, linearity range, reproducibility, etc. are established for the known bioactive compound(s) of crude drugs  (e.g. herbal drugs), a concentration-response profile is constructed from which the presence and/or amount of active ingredient(s) in drugs can be accurately measured.

The figure above shows the HPLC profile of a known drug-standard tested at different concentrations. The peak area of each determination is then plotted against the response (e.g. UV absorption). A straight line equation with statistical significance must then be plotted from which the concentration of the bioactive compound(s) in the test sample  is established. We use a minimum of SIX determinations to ascertain accuracy/precision.

Contact us if you want  expert advice, consultancy and/or services in this area.

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