ANTIOXIDANT ACTIVITY TEST
Reactive oxygen species (ROS)
such as hydrogen peroxide, superoxide anion and hydroxyl radicals are
continuously formed during normal physiological processes (e.g. mitochondrial respiratory
chain) and also by exogenous factors (e.g.
toxins). Under normal conditions, the level of these species are kept
checked by our antioxidant defences and hence only their important physiological
roles such as in cellular/subcellular signalling is noticed. Under abnormal
physiological conditions, however, the production of ROS formation may exceed their
destruction and lead to the development of pathological conditions. Cancer,
disease and inflammatory disorders are among the few
disease conditions associated with ROS. Natural antioxidants that
either reduce the formation of ROS, those that directly neutralise ROS or those
with potential for enhancing our antioxidant defences are thus required to
combat diseases mediated by ROS. For many decades, there has also been a
growing interest in finding natural antioxidants that could safely prolong the
shelf-life of foods and uses as as nutraceuticals.
In our laboratories, we routinely
screen plant-derived potential antioxidants by using the following models:
1. Chemical assays that measure the
scavenging potential of test agents against ROS.
2. Assays based on the interaction of
ROS with key biological molecules such as DNA, proteins and lipids.
3. Bioassays that measure potential
antioxidant activity in a cellular system.
For any further information on the
subject or collaborative and/or consultancy work please
contact the site owner.
You may also find the following key publications of interest:
HABTEMARIAM, S.*, Varghese, G.K. (2017).
Antioxidant, anti-alpha-glucosidase and
pancreatic beta-cell protective effects
of methanolic extract of
Asian Pacific Journal of Tropical
HABTEMARIAM, S., Di Lorenzo, A., Sureda,
A., Khanjani, S., Nabavi, S.M., Daglia,
M. (2016). Post-stroke depression
modulation and in vivo antioxidant
activity of gallic acid and its
synthetic derivatives in a murine model
8(5), 248; doi:10.3390/nu8050248
Novel Diterpene Skeleton: Identification
highly aromatic, cytotoxic and
Curti, V., Capelli, E., Nabavi,
S.F., HABTEMARIAM, S.,
Daglia, M. (2014).
Modulation of human miR-17-3p
expression by methyl 3-O-methyl
gallate as explanation of its in vivo
protective activities. Molecular
Nutrition & Food Research
S., Nabavi, S.M. (2014). Curcumin
and liver health, from chemistry to
Comprehensive Reviews in Food Science
and Food Safety
Nabavi, S.M., HABTEMARIAM, S., Nabavi,
S.F., Sureda, A., Moghaddam, A.H., Latifi, A.M. (2013). Prophylactic effect
of methyl-3-O-methyl gallate
against sodium fluoride-induced oxidative stress in rat’s erythrocytes
Journal of Pharmacy and Pharmacology
65(6), 868-873. .Abstract
Nabavi, S.F., Nabavi, S.M.,
HABTEMARIAM, S., Sureda, A., Moghaddam, A.H., Latifi, A.M. (2013).
Neuroprotective effects of methyl-3-O-methyl
gallate against sodium fluoride-induced oxidative stress in brain of rats.
Cellular and Molecular Neurobiology
HABTEMARIAM, S.*, Sureda, A., Moghaddam, A.H., Nabavi,
S.F., Nabavi, S.M., Abolhasani, F. (2013). Protective role of gallic acid isolated from
Peltiphyllum peltatum against
sodium fluoride-induced oxidative stress in rat’s heart.
Letters in Drug Design and Discovery
Bose, L.V., Varghese, G.K.,
HABTEMARIASM, S*. (2013). Identification of acteoside as the active
antioxidant principle of
root wood tissues.
Full Text Article
Nabavi, S.F., Nabavi, S.M., HABTEMARIAM,
S., Sureda, A., Jafari, M., Abolhasani,
F., Moghaddam, A.H. (2013).
Hepatoprotective effect of gallic acid
Peltiphyllum peltatum against
sodium fluoride-induced oxidative
Industrial Crops and Products
abavi, S.F., Nabavi, S.M., HABTEMARIAM,
M., Moghaddam, A.H. (2012).
Protective effect of methyl-3-O-methyl
gallate against sodium fluoride-induced
oxidative stress in rat’s cardiac
45(3 Pt 2),
Nabavi S.M., Latifi A.M., Mirzaei M., HABTEMARIAM, S.,
Moghaddam, A.H. (2012).
Mitigating role of
quercetin against sodium fluoride induced oxidative stress in rat’s brain.
Nabavi S.M., Nabavi, S.F., HABTEMARIAM, S., Moghaddam,
A.H., Latifi, A.M. (2012). Ameliorative effects of quercetin on sodium
fluoride-induced oxidative stress in rat's kidney.
Nabavi, S.F., HABTEMARIAM, S., Jafari, M, Sureda, A.,
Nabavi S.M. (2012). Protective role of gallic acid on sodium fluoride
induced oxidative stress in rat brain. Bulletin of Environmental
Contamination and Toxicology.
HABTEMARIAM, S. (2012). Antihyperlipidemic components of
aerial parts: Identification
HABTEMARIAM, S*. and
Cowley R.A. (2012).
compounds from the
Peltiphyllum peltatum (Torr.) Engl.
Roselli, M., Lentini, G. and HABTEMARIAM, S*. (2012).
Phytochemical, antioxidant and anti-alpha-glucosidase
activity evaluations of Bergenia cordifolia.
Roselli, M., Lovece, A., Bruno, C., Lentini, G., Corbo,
F., la Forgia F., Fontana, S., HABTEMARIAM, S., Franchini, C. (2012). Le
Proprieta antiossidanti dell’uva di Troia Canosina ad acino piccolo:
confronto tra differenti metodi
Juan-Badaturuge, M., HABTEMARIAM, S., Thomas, M.J.K. (2011).
Antioxidant compounds from a South Asian beverage and medicinal plant,
Cassia auriculata. Food Chemistry
Wadegaonkar , P.A. (2010). Antioxidant and
antiproliferative activity of root suspension culture of
Research Journal of Pharmacy & Technology
HABTEMARIAM S. and Dagne E. (2010).
Comparative antioxidant, prooxidant and cytotoxic activity of sigmoidin A
and eriodictyol. Planta Med.
Juan-Badaturugea M., HABTEMARIAM S., Jackson C., and
Thomas MJK. (2009). Antioxidant principles of Tanacetum vulgare L. aerial part. Natural
Product Communications 4(11),
HABTEMARIAM, S. and Dagne,
E. (2009). Prooxidant action of knipholone anthrone: Copper dependent
reactive oxygen species generation and DNA damage.
Food and Chemical
Antioxidant and antiinflammatory effect of Epilobium parviflorum
Phytother. Res. 23(5), 719-724.
(2008). Activity-guided Isolation and Identification of
Free Radical-scavenging Components from Ethanolic
Extract of Boneset (Leaves of
Natural Product Communications
Activity-guided Isolation and Identification of
Antioxidant Components from Ethanolic Extract of
Natural Product Communications
HABTEMARIAM, S., Houghton, P (2008). Free radical
scavenging activity of crude extracts and
4’-O-Methyl-(-) epigallocatechin isolated from roots of
Cassine transvaalensis from Botswana.
African Journal of
Biomedical Research 11, 55-63
HABTEMARIAM, S. (2007).
Antioxidant activity of Knipholone anthrone.
HABTEMARIAM, S. and Jackson, C. (2007).
Antioxidant and cytoprotective activity of
leaves of Peltiphyllum peltatum (Torr.) Engl.
Motlhanka. D.M.T., Miljkovic-Brake,
A., Houghton, P.J., HABTEMARIAM, S., Hylands, P.J.
(2006). Antioxidant activity of water and ethanol
extracts from Roots of Cassine transvaalensis Burtt-Davy from Botswana.
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The Site Owner
Dr Habtemariam BSc, MSc,
PhD........FRSM, FRSC. .... With a
Principal Lecturer post at Greenwich, Dr Habtemariam
is a leader of the BSc Pharmaceutical Science programme and
researches on bioassay & natural products-based drug
addition to our fully equipped tissue culture
facilities, we have access to various
state-of-the-art equipment including ICP-MS, LC-MS,
MALDI-TOF-MS, FABS-MS, FTIR, Laser-Raman,
scanning and transmission electron
microscopes, flow cytometer, NMR (270, 300
and 500MHz), automated DNA sequencers, various HPLC
systems, capillary electrophersis and ABI PRCmate DNA